HyTest anti-nucleoprotein antibodies expected to detect both UK and South African variants of SARS-CoV-2 3CV4 Anti-Nucleoprotein MAbs
The UK variant of SARS-CoV-2 (known as VOC 202012/01, lineage B.1.1.7, or 20B/501Y.V1) has four mutations in the nucleoprotein: D3L, R203K, G204R and S235F. We have confirmed that all our anti-SC-NP clones recognize both the wild type and UK variant nucleoprotein (recombinant).
The South African variant of SARS-CoV-2 (known as 501.V2, 20C/501Y.V2 or B.1.351 lineage) does not have any mutations in its nucleoprotein and is thus recognized similarly to the wild type virus.3CV2 Anti-Spike MAbs
The UK variant has deletions and mutations within the spike protein, one of which is within the RBD part of the protein (N501Y). We have confirmed that all our anti-RBD clones detect the N501Y variant (recombinant antigen) similarly to the wild type virus.
The South African variant bears four mutations in its spike protein (K417N, E484K, N501Y, D614G). Our anti-Spike clones recognize the variant spike protein similarly to wild type virus.
Figure 1. Recognition of the recombinant variant antigens with HyTest anti-nucleoprotein and anti-spike antibodies. Signal for wild type rec. antigen = 100%.
We recommend using more than two clones in assay development
Due to the continuing mutation of SARS-CoV-2 and spread of new variants in different countries we recommend using more than two clones in tests. This will increase the likelihood of a SARS-CoV-2 test to detect the different variants also in the future.
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