GAPDH - a Loading Control in Western Blotting

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is one of the key enzymes involved in glycolysis. It is one of the so called housekeeping proteins and GAPDH is constitutively expressed in almost all tissues in high amounts. For this reason, GAPDH is widely used as a loading control for protein normalization in Western blotting. It is also useful for cell visualization in microscopy assays and can be used to investigate cell metabolism and functional activity.

We offer monoclonal antibodies specific to GAPDH and native GAPDH proteins purified from human and rabbit tissues. The antibodies cross-react with GAPDH in several animal species (see Table). For more information, please see our GAPDH TechNotes under the “Literature” tab.

Table. Cross-reactivity of anti-GAPDH monoclonal antibodies (Cat.# 5G4) with different species.

TechNotes

GAPDH TechNotes