DATA SHEET

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PRODUCT NAME: Thermus aquaticus (Taq) DNA polymerase enhancer (DPE)
CATALOGUE #: 7T3

Description:
Taq DPE is an additive that can greatly increase the yield and specificity of primer extension reactions. DPE destabilizes many mismatched primer-template complexes that would otherwise result in a heterogeneous population of molecules. The addition of DPE generates greater yields in case of limited amounts of amplified product using standard conditions, as well as results in a substantial increase in PCR product specificity.

Storage and dilution buffer:
25 mM Tris-HCl, pH 8.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.75 % Triton X-100, 50 % glycerol.

Reaction conditions:
Add 1-2 u of DPE in master mix (without template DNA), incubate 5-10 min at room temperature and then add DNA.

Applications:
PCR pattern improvement, PCR diagnostic and DNA sequencing improvement.

Quality control:
Absence of endonucleases/nickases and exonucleases.

Concentration:
1-2 units/µl

Storage:
-20 °C