Unipol Polymerase
DATA SHEET
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Unipol polymerase
Cat.# 7U1
Description:
Unipol polymerase is a special mixture of thermostable enzymes purified from recombinant strains expressing Thermus polymerase gene.
Unipol polymerase also exhibits reverse transcription activity in presence of Mn 2+ ions, in much higher concentration than that demonstrated for Taq DNA polymerase.
The presence of thermostable pyrophosphatase (PPase-t) in the mix reduces the concentration of organic pyrophosphate (PPi), which accumulates during incorporation of nucleotide triphosphates (dNTP's) and inhibits DNA polymerase. An addition of PPase-T results in significant improvement in DNA amplification; it improves yield and reduces background. Optimal ratio of all components of the Unipol polymerase gives an opportunity to amplify long templates (up to 15 Kb) and increase the number of effective amplification cycles (up to 45-50), which is very important for low-copy number DNA templates and amplification of large fragments.
Storage and dilution buffer:
10 mM K-phosphate buffer (pH 7.0), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 50 % glycerol, 0.1 mg/ml BSA.
Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 74 oC.
Amplification buffer 10x:
NH4-buffer: 166 mM (NH4)2SO4, 670 mM Tris-HCl (pH 8.8 at 25 °C), 0.1 % Tween 20.
Chelating buffer 5x:
166 mM (NH4)2SO4, 670 mM Tris-HCl (pH 8.8 at 25 °C), 7.5 mM EGTA, 0.25 % Tween-20, 50 % glycerol.
Applications:
PCR, RT-PCR, low-copy targets PCR.
Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Concentration:
5 units/µl
Storage:
-20 °C
Additional product information:
Enzymes and Reagents for molecular biology TechNotes
