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Troponin I Cardiac antibody
Printable version: [ Troponin I Cardiac antibody ]
DATA SHEET This data sheet is provided for information only. Please contact our Customer Service for further information. PRODUCT NAME: Monoclonal mouse anti-cardiac troponin I (cTnI) CATALOGUE #: 4T21 MAbs: 8E10, C5, 4C2, 19C7, 16A11, 10B11, 3C7, 23C6, P4-14G5, M18, M155, M46, MF4, 16A12, P4-9F6, 228, 625, 458, 267, 596, 84, 415, 581, 441 New MAbs: 10F4, 247, 560, 17F3, p45-10 Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI, native human cTn complex, or human cTnI peptides. Specificity: For epitope specificity see printable version. All MAbs (except M46, 267, 596 and C5) have no cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10 % for MAbs M46, 267 and 596, and more than 50 % for MAb C5. MAb 247 reacts only with free cTnI. Additional comprehensive information about cross-reactivity with different forms of antigens (phosphorylated, complexed etc.) and antigens from different species (rat, mouse, canine, bovine and porcine) is available. Please inquire also HyTest Markers of Cardiovascular Diseases catalogue. MAb isotypes: IgG for MAb p45-10 IgG1 for MAbs 8E10, 16A11, 3C7, 10B11, P4-14G5, M18, M155, M46, MF4, P4-9F6, 228, 625, 560, 596, 16A12, 441, 415, 84, 581, 247, 17F3 IgG2a for MAbs 4C2, 23C6, 267, 10F4 IgG2b for MAbs 19C7 and C5 IgM for MAb 458 Applications: cTnI immunoassay; cTnI immunoprecipitation, immunohistochemistry, immunoaffinity purification. All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using MAbs 19C7, 16A11 and MF4. Recommended pairs for quantitative sandwich immunoassay (coating - conjugate): 625 - 19C7, 560 - 458. Best pairs: 19C7 - 16A11, 560 - 19C7 Antibody combinations for quantitative sandwich immunoassay: 19C7 + M18 (mixture for coating) - MF4 + 16A11 (mixture for detection) M18 + 560 (mixture for coating) - MF4 + 19C7 (mixture for detection) 228 + 560 (mixture for coating) - 19C7+ 458 (mixture for detection) M18 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection) MF4 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection) 19C7 + MF4 (mixture for coating) - 7B9 (anti-TnC MAb; Cat. # 4T27) Purification: Chromatography on protein A Sepharose Presentation: PBS, pH 7.4, 0.1 % sodium azide (NaN3) Storage: + 4 °C Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. Related products: Cat# 4T45: Troponin I Cardiac, Phosphorylated, antibody Cat# 4T46: Troponin I Cardiac, Dephosphorylated, antibody Cat# 8T53: Troponin I Cardiac, Human Cat# 8T53b: Troponin I Cardiac, Bovine Cat# 8T53c: Troponin I Cardiac , Canine Cat# 8T53dp: Troponin I Cardiac, Dephosphorylated Cat# 8T53m: Troponin I Cardiac, Mouse Cat# 8T53p: Troponin I Cardiac, Porcine Cat# 8T53ph: Troponin I Cardiac, Phosphorylated Cat# 8T53r: Troponin I Cardiac, Rat Cat# 8T53rb: Troponin I Cardiac, Rabbit Cat# 8T60: Troponin I Cardiac Calibrator Set* Cat# 8TFS: Troponin I free serum Cat# K01: Troponin I Cardiac Diversity Kit Cat# 8T62: Troponin Complex (I-T-C), Human Cat# 8T62a: Troponin Complex (I-T-C), artificial Cat# 8T62b: Troponin Complex (I-T-C), Bovine Cat# 8T62c: Troponin Complex (I-T-C), Canine Cat# 8T62m: Troponin Complex (I-T-C), Mouse Cat# 8T62p: Troponin Complex (I-T-C), Porcine Cat# 8T62rb: Troponin Complex (I-T-C), Rabbit Cat# 8T62r: Troponin Complex (I-T-C), Rat Cat# 8T57: Troponin C Cardiac Cat# 8T57b: Troponin C cardiac Bovine Cat# 8T57c: Troponin C cardiac Canine Cat# 8T57m: Troponin C cardiac Mouse Cat# 8T57p: Troponin C Cardiac Porcine Cat# 8T57rb: Troponin C cardiac, Rabbit Cat# 8T57r: Troponin C cardiac, Rat Cat# 4T19: Troponin T Cardiac antibody Cat# 8T13: Troponin T Cardiac, Human Cat# 8T13b: Troponin T Cardiac, Bovine Cat# 8T13c: Troponin T Cardiac, Canine Cat# 8T13m: Troponin T Cardiac, Mouse Cat# 8T13p: Troponin T Cardiac, Porcine Cat# 8T13rb: Troponin T cardiac, Rabbit Cat# 8T24: Troponin T Skeletal Muscle - Human Cat# 8T24b: Troponin T Skeletal Muscle, Bovine Cat# 8T24c: Troponin T Skeletal Muscle - Canine Cat# 8T24m: Troponin T Skeletal Muscle - Mouse Cat# 8T24p: Troponin T Skeletal Muscle, Porcine Cat# 8T24rb: Troponin T Skeletal Muscle, Rabbit Troponin I Newsletter: Concept of precise immunoassay See other cardiac and blood coagulation products Publications: Search Troponin I references from PubMed. See other References [back]

Troponin I Cardiac antibody

Printable version: [ Troponin I Cardiac antibody ]

DATA SHEET

This data sheet is provided for information only.
Please contact our Customer Service for further information.

PRODUCT NAME: Monoclonal mouse anti-cardiac troponin I (cTnI)
CATALOGUE #: 4T21

MAbs:
8E10, C5, 4C2, 19C7, 16A11, 10B11, 3C7, 23C6, P4-14G5, M18, M155, M46, MF4, 16A12, P4-9F6, 228, 625, 458, 267, 596, 84, 415, 581, 441
New MAbs: 10F4, 247, 560, 17F3, p45-10
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI, native human cTn complex, or human cTnI peptides.

Specificity:
For epitope specificity see printable version.
All MAbs (except M46, 267, 596 and C5) have no cross-reaction with skeletal muscle TnI. Cross-reactivity with skeletal TnI is less than 10 % for MAbs M46, 267 and 596, and more than 50 % for MAb C5. MAb 247 reacts only with free cTnI.
Additional comprehensive information about cross-reactivity with different forms of antigens (phosphorylated, complexed etc.) and antigens from different species (rat, mouse, canine, bovine and porcine) is available. Please inquire also HyTest Markers of Cardiovascular Diseases catalogue.

MAb isotypes:
IgG for MAb p45-10
IgG1 for MAbs 8E10, 16A11, 3C7, 10B11, P4-14G5, M18, M155, M46, MF4, P4-9F6, 228, 625, 560, 596, 16A12, 441, 415, 84, 581, 247, 17F3
IgG2a for MAbs 4C2, 23C6, 267, 10F4
IgG2b for MAbs 19C7 and C5
IgM for MAb 458

Applications:
cTnI immunoassay; cTnI immunoprecipitation, immunohistochemistry, immunoaffinity purification.
All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using MAbs 19C7, 16A11 and MF4.

Recommended pairs for quantitative sandwich immunoassay (coating - conjugate):
625 - 19C7, 560 - 458. Best pairs: 19C7 - 16A11, 560 - 19C7

Antibody combinations for quantitative sandwich immunoassay:
19C7 + M18 (mixture for coating) - MF4 + 16A11 (mixture for detection)
M18 + 560 (mixture for coating) - MF4 + 19C7 (mixture for detection)
228 + 560 (mixture for coating) - 19C7+ 458 (mixture for detection)
M18 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection)
MF4 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection)
19C7 + MF4 (mixture for coating) - 7B9 (anti-TnC MAb; Cat. # 4T27)

Purification:
Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

See other cardiac and blood coagulation products

Publications:
Search Troponin I references from PubMed.
See other References

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Troponin I Cardiac antibody

DATA SHEET

PRODUCT NAME: Monoclonal mouse anti-cardiac troponin I (cTnI) CATALOGUE #: 4T21

PRODUCT NAME: Monoclonal mouse anti-cardiac troponin I (cTnI)
CATALOGUE #: 4T21