Troponin I Cardiac antibody

DATA SHEET

This data sheet is provided for information only.
Please contact our Customer Service for further information and pricing.

Open the printable PDF-file

Monoclonal mouse anti-cardiac troponin I (cTnI)
Cat.# 4T21

MAbs:
8E10, C5, 4C2, 19C7, 16A11, 10B11, 3C7, 23C6, P4-14G5, M18, M155, M46, MF4, 16A12, P4-9F6, 228, 625, 458, 267, 596, 84, 415, 581, 441
New MAbs: 10F4, 247, 560, 17F3, p45-10, 472
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cardiac troponin I, native human cardiac troponin complex, or human cardiac troponin I peptides.

Specificity:
For epitope specificity see the printable version.
None of the MAbs (except M46, 267, 596, 472 and C5) have no cross-reaction with skeletal muscle troponin I. Cross-reactivity with skeletal troponin I is less than 10% for MAbs M46, 267 and 596, and more than 50% for MAbs C5 and 472. MAb 247 reacts only with free cardiac troponin I.
Additional comprehensive information about cross-reactivity with different forms of antigens (phosphorylated, complexed etc.) and antigens from different species (rat, mouse, canine, bovine and porcine) is available. Please inquire also HyTest Markers of Cardiovascular Diseases catalogue.

MAb isotypes:
IgG for MAb p45-10
IgG1 for MAbs 8E10, 16A11, 3C7, 10B11, P4-14G5, M18, M155, M46, MF4, P4-9F6, 228, 625, 560, 596, 16A12, 441, 415, 84, 581, 247, 17F3
IgG2a for MAbs 4C2, 23C6, 267, 10F4
IgG2b for MAbs 19C7 and C5
IgG3 for MAb 472
IgM for MAb 458

Applications:
Cardiac troponin I immunoassay; cardiac troponin I immunoprecipitation, immunohistochemistry, immunoaffinity purification.
All MAbs work in Western blotting. For better sensitivity in Western blotting we recommend using MAbs 19C7, 16A11 and MF4.

Recommended pairs for quantitative sandwich immunoassay (coating - conjugate): 
625 - 19C7, 560 - 458. Best pairs: 19C7 - 16A11, 560 - 19C7

Antibody combinations for quantitative sandwich immunoassay:
19C7 + M18 (mixture for coating) - MF4 + 16A11 (mixture for detection)
M18 + 560 (mixture for coating)  - MF4 + 19C7 (mixture for detection)
228 + 560 (mixture for coating) - 19C7+ 458 (mixture for detection)
M18 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection)
MF4 + 560 (mixture for coating) - 19C7 + 267 (mixture for detection)
19C7 + MF4 (mixture for coating) - 7B9 (anti-TnC MAb; Cat. # 4T27)

Purification:
Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN₃)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:
Troponin I TechNotes

References:
Search Troponin I references from PubMed.
See all Troponin I References
See references where Monoclonal mouse anti-cardiac troponin I (cTnI) has been used in

Related products:
Cat# 4T45: Troponin I Cardiac, Phosphorylated, antibody   
Cat# 4T46: Troponin I Cardiac, Dephosphorylated, antibody 
Cat# 8T53: Troponin I Cardiac, Human  
Cat# 8T53b: Troponin I Cardiac, Bovine
Cat# 8T53c: Troponin I Cardiac , Canine
Cat# 8T53dp: Troponin I Cardiac, Dephosphorylated 
Cat# 8T53m: Troponin I Cardiac, Mouse  
Cat# 8T53p: Troponin I Cardiac, Porcine  
Cat# 8T53ph: Troponin I Cardiac, Phosphorylated  
Cat# 8T53r: Troponin I Cardiac, Rat  
Cat# 8T53rb: Troponin I Cardiac, Rabbit  
Cat# 8T60: Troponin I Cardiac Calibrator Set*  
Cat# 8TFS: Troponin I free serum
Cat# K01: Troponin I Cardiac Diversity Kit
Cat# 8T62: Troponin Complex (I-T-C), Human
Cat# 8T62a: Troponin Complex (I-T-C), artificial 
Cat# 8T62b: Troponin Complex (I-T-C), Bovine
Cat# 8T62c: Troponin Complex (I-T-C), Canine    
Cat# 8T62m: Troponin Complex (I-T-C), Mouse
Cat# 8T62p: Troponin Complex (I-T-C), Porcine  
Cat# 8T62rb: Troponin Complex (I-T-C), Rabbit  
Cat# 8T62r: Troponin Complex (I-T-C), Rat
Cat# 8T57: Troponin C Cardiac  
Cat# 8T57b: Troponin C cardiac Bovine  
Cat# 8T57c: Troponin C cardiac Canine  
Cat# 8T57m: Troponin C cardiac Mouse  
Cat# 8T57p: Troponin C Cardiac Porcine  
Cat# 8T57rb: Troponin C cardiac, Rabbit  
Cat# 8T57r: Troponin C cardiac, Rat
Cat# 4T19: Troponin T Cardiac antibody
Cat# 8T13: Troponin T Cardiac, Human
Cat# 8T13b: Troponin T Cardiac, Bovine
Cat# 8T13c: Troponin T Cardiac, Canine   
Cat# 8T13m: Troponin T Cardiac, Mouse    
Cat# 8T13p: Troponin T Cardiac, Porcine  
Cat# 8T13rb: Troponin T cardiac, Rabbit
Cat# 8T24: Troponin T Skeletal Muscle - Human  
Cat# 8T24b: Troponin T Skeletal Muscle, Bovine 
Cat# 8T24c: Troponin T Skeletal Muscle - Canine
Cat# 8T24m: Troponin T Skeletal Muscle - Mouse  
Cat# 8T24p: Troponin T Skeletal Muscle, Porcine  
Cat# 8T24rb: Troponin T Skeletal Muscle, Rabbit 

See other cardiac and blood coagulation products