DATA SHEET

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PRODUCT NAME: Human cardiac troponin I (cTnI)
CATALOGUE #: 8T53

Source:
Human heart tissue.
Blood sample from the tissue donors was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.

Applications:
TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity.
TnI exists in three isoforms, one for slow-twitch skeletal muscle, one for fast twitch skeletal muscle and one for cardiac muscle. The amino acid sequences of two skeletal (skTnI) and one cTnI forms how 40% dissimilarity. Furthermore, the human cTnI has additional amino acid residues on its N-terminal region that are not present on the skeletal form, making this protein a promising analyte for indicating cardiac specificity. cTnI follows similar release pattern as CK-MB within the first 15 hours of myocardial infarction (MI), but remains elevated in serum up to 6 days. This observation and the unique amino sequence of cTnI make it an important diagnostic marker of MI.
TnI is suitable for use as a standard in immunoassay for early detection of MI, immunogen for antiserum production and tracer for iodination.

Analysis:
Purity > 98 % (SDS-PAGE).
Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.
TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).

Presentation:
Freeze-dried from 0.01 M HCl.
Product is recommended to reconstitute with urea/Tris buffer, pH 7.5 (7 M urea, 5 mM EDTA, 15 mM mercapthoethanol, 20 mM Tris) to concentration around 1mg/ml.
Solubility is limited at neutral pH and physiological salt concentration.

Storage:
- 20 °C (- 70 °C for long term storage)

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References:
Search Troponin I references from PubMed.
See other References