Streptavidin Streptomyces avidinii, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-Streptavidin from
Streptomyces avidinii
Cat.# 3ST10
MAbs:
S8E4, S8C12, S10D4, S3E11
Specificity:
No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAb S8C12 reacts with the determinant nonoverlapping with S8E4-, S10D4- and S3E11-epitopes. All MAbs bind to the streptavidin without interfering with the biotin-binding reaction.
MAb isotypes:
IgG1, kappa for all MAbs
Applications:
MAbs can be used to localize streptavidin, to amplify signals in immunochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
