SmarTaq Polymerase
DATA SHEET
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SmarTaq Polymerase
Cat.# 7S1
Description:
SmarTaq DNA Polymerase is complex mixture of a thermostable 94 kDa BioTaq DNA Polymerase purified from E. coli PVG-AI recombinant strain expressing Thermus aquaticus polymerase gene and specific monoclonal antibodies.
SmarTaq is inactive under conditions of amplification reaction preparation. It provides improved specificity when compared to standard DNA polymerases. SmarTaq can eliminate amplification artefacts such as primer-dimer formation and mis-priming.
An advantage of SmarTaq is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An active complex of SmarTaq dissociates automatically over +70 °C, allowing activation of DNA polymerase.
Storage and dilution buffer:
20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50 % glycerol, 0.5 % Nonidet P-40, 0.5 % Tween-20.
Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 74 °C.
Amplification buffer 10x:
NH4-buffer: 166 mM (NH4)2SO4, 670 mM Tris-HCl (pH 8.8 at 25 °C), 0.1 % Tween-20.
Applications:
Highly specific PCR, multiplex PCR (highly recommended), high sensitivity applications.
Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Concentration:
5 units/µl
Storage:
-20 °C
Additional product information:
Enzymes and Reagents for molecular biology TechNotes
References:
See references where SmarTaq Polymerase has been usen in
