PSA, Human, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-prostate specific antigen (PSA)
Cat.# 4P33
MAbs: 2H9, 5A6, 8A6, 5G6, PS1, PS2, PS6
New MAb: 1H12
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.
Specificity:
See the printable version for information.
MAb isotypes:
IgG1 for MAbs 5G6, PS6, PS2, PS1
IgG2a for MAbs 2H9, 5A6, 8A6, 1H12
Applications:
Detection of PSA antigen. Recommended pairs for detection of total PSA (coating - conjugate):
5A6-2H9, PS2 - PS6, 5G6 - 5A6. Best pair: 1H12- 5A6
Recommended pairs for detection of free PSA (coating - conjugate):
5A6-8A6, 2H9-8A6, PS2-PS1, PS1-PS2, 8A6 - 5A6. Best pair: 8A6 - 5A6
MAbs can also be used in lateral flow.
Purification:
MAbs PS1, PS2 and PS6 by ion exchange chromatography and other MAbs by chromatography on protein A Sepharose.
Presentation:
MAbs 2H9, 5A6, 8A6, 5G6 and 1H12 in PBS, pH 7.4, 0.1 % sodium azide (NaN3).
MAbs PS1, PS2 and PS6 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % of sodium azide (NaN3).
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.05 - 0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
