Proinsulin, human, antibody
DATA SHEET
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Monoclonal mouse anti-human proinsulin
Cat.# 2P9
MAbs:
3A1, 1G4, 1D6, 1D3, 7D6
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human proinsulin.
Specificity:
MAbs 3A1 and 1G4 are directed to different antigenic determinants. MAb 3A1 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.
MAbs 1D6 and 1D3 react with different epitopes on human proinsulin molecule; both of each are generated by the interactions between insulin and C-peptide parts of prohormone.
Kd for MAb 3A1 is 1.1 x 10-8 M.
Kd for MAb 1G4 is 2.6 x 10-7 M.
Kd for MAb 1D6 and 1D3 is >3 x 10-8 M.
Kd for MAb 7D6 is > 5 x 10-8 M.
MAbs 3A1, 1G4, 1D6, 1D3 and 7D6 do not cross-react with human, bovine and porcine insulin, bovine and porcine proinsulin, and human C-peptide.
MAb isotypes:
IgG1, kappa for all MAbs
Applications:
Detection of human proinsulin in two-site enzyme immunoassay.
Recommended pairs for sandwich immunoassay (coating - conjugate):
3A1 - 7F8 (Cat.# 2I1)
1G4 - mixture of D6C4 and D3E7 (Cat.# 2IP10)
1D6 or 1D3 - 7F8, 8E2 (Cat.# 2I1), D6C4 or D3E7 (Cat.# 2IP10) (Could be used for direct coating of prohormone in serum samples)
MAbs 3A1 and 1G4 can be used in immunohistochemistry on frozen sections.
Specific immunosorbtional removal of proinsulin molecules (with immobilized 1D6/1D3 MAbs) from serum samples before the measurement of insulinic C-peptide.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:
Rat C-peptide and proinsulin TechNotes
References:
Search Proinsulin references from PubMed
See all Insulin, Proinsulin, C-peptide References
See references where Monoclonal mouse anti-human proinsulin has been used in
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Cat# 2I1: Insulin, human, antibody
Cat# 2IP10: Insulin-Proinsulin, rat-mouse, antibody
Cat# 2PR8: Rat proinsulin antibody
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