Progesterone antibody

DATA SHEET

This data sheet is provided for information only.
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Monoclonal mouse anti-progesterone
Cat.# 2P2

MAbs:
XM207, HPRO-1, HPRO-2, HPRO-3
Hybridoma clone for MAb XM207 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified progesterone with BSA. For HPRO-1, HPRO-2 and HPRO-3 immunogen was conjugate of 17α-hydroxyprogesterone with BSA.

Specificity:
MAb XM207 - Progesterone, Ka = 3.0 x 10⁹ M^-1
MAb HPRO-2 - Progesterone
MAbs HPRO-1,  HPRO-3 - 17α-hydroxyprogesterone
MAb XM207 reacts with progesterone in ELISA. MAbs HPRO-1, HPRO-2 and HPRO-3 react with 17α-hydroxyprogesterone-BSA conjugate and free 17α-hydroxyprogesterone. There is no cross-reactivity with BSA.
For further cross-reactivity information see the printable version.

MAb isotypes:
IgG2b for MAbs XM207, HPRO-2 and HPRO-3
IgG3 for MAb HPRO-1

Applications:
XM207 and HPRO-2 can be used for detection of progesterone.
HPRO-1 and HPRO-3 can be used in competitive assay of 17α-hydroxyprogesterone.

Purification:
HPRO-1, HPRO-2 and HPRO-3 by chromatography on protein G Sepharose, and XM207 by ion exchange chromatography.

Presentation:
HPRO-1, HPRO-2 and HPRO-3 in PBS, pH 7.4, 0.1 % sodium azide (NaN3),
XM207 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% of sodium azide (NaN3).

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.