Plasminogen, Human, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human plasminogen
Cat.# 4P11
MAbs:
4D2, 9E7, 5H3, 9D8, 8E7, 8F11, 3C2
Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with purified human plasminogen.
Specificity:
MAbs 4D2 and 9E7 react with human plasminogen.
MAbs 9D8, 8E7 and 3C2 react with human plasminogen and plasmin molecules.
MAbs 5H3 and 8F11 react with plasminogen, plasmin and angiostatin.
No cross-reactivity with other human serum proteins.
MAb isotypes:
IgG1 for MAbs 5H3, 8E7, 8F11 and 3C2
IgG2a for MAbs 9E7 and 9D8
IgG2b for MAb 4D2
Applications:
Affinity purification of human plasminogen.
Detection of plasminogen in ELISA and immunohistochemistry.
All MAbs work in immunohistochemistry on formalin fixed and paraffin embedded sections.
Optimal dilution must be determined by experimentation.
Recommended pairs for sandwich immunoassay (coating - conjugate): 8F11 - 8E7 and 5H3 - 8E7
Purification:
Ion exchange chromatography
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
