DATA SHEET

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PRODUCT NAME: Human recombinant NT-proBNP (amino terminal human brain natriuretic peptide precursor)
CATALOGUE #: 8NT2

Source:
Recombinant E.coli

Application: 
Calibrator or standard in immunoassays, immunogen for antibody production.

Analysis:
Purity > 95 % (Tricine-SDS-PAGE)
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).

Purification: 
Immunoaffinity purification followed by ion-exchange chromatography.

Presentation:
10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

Storage:
- 70 °C

Other information:
Avoid repeated freezing and thawing.
It is recommended to aliquot the product after the first thawing.

To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).

Some applications in which this product may be used are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license, depending on the particular application and country in which the product is used.

Related products:
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Cat# 4BFab5: Immune complex 24C5-BNP_proBNP, antibody 
Cat# 4NT1: NT-proBNP, human, antibody  
Cat# 8NT1: NT-proBNP recombinant   
Cat# 8PRO9: proBNP, recombinant, human 
Cat# 8PRO8: proBNP human recombinant   
Cat# 8GOB2: Human recombinant proBNP glycosylated
Cat# 8BFP: BNP and NT-proBNP Free Plasma
ProBNP, BNP and NT-proBNP Newsletter 

See other Cardiac markers and blood coagulation products

References:
Search NT-proBNP recombinant references from PubMed
See other NT-pro BNP References