DATA SHEET

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PRODUCT NAME: Monoclonal mouse anti- human N-terminal proBNP (NT-proBNP)
CATALOGUE #: 4NT1

MAbs:
5B6, 15F11, 7B5, 13G12, 11D1, 16E6, 15D7, 15C4, 24E11, 28F8, 29D12, 18H5, 16F3
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 1-12 (MAbs 5B6, 29D12), a.a.r. 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3), a.a.r. 28-45 (MAbs 11D1, 16E6), a.a.r. 46-60 (MAb 15D7) and a.a.r. 61-76 (MAbs 24E11, 28F8, 15C4). The peptides used for immunization were conjugated with carrier protein.

Specificity:
Human NT-proBNP and proBNP.

MAb isotypes:
IgG1 for MAbs 11D1, 5B6, 15D7, 7B5, 16E6, 18H5, 16F3
IgG2a for MAbs 29D12, 13G12, 24E11, 28F8
IgG2b for MAbs 15F11, 15C4

Applications:
NT-proBNP and proBNP immunoassay. All MAbs are working in Western Blotting.
Best pairs for sandwich immunoassay (coating - conjugate): 
15F11 - 24E11, 15C4 - 29D12, 15C4 - 13G12, 15C4 - 18H5. 
These pairs recognize the antigen in blood of patients with heart failure, unstable angina and myocardial infarction.
MAbs specific to peptides 5-12 (MAb 29D12) and 13-27 (MAbs 15F11, 13G12, 18H5, 7B5, 16F3) recognize circulating proBNP and can be used for the development of quantitative proBNP assays in pairs with anti-BNP MAbs (Cat.# 4BNP2). The best pairs (coating - conjugate):
50E1 (Cat.# 4BNP2) - 16F3 (Cat.# 4NT1)
50E1 (Cat.# 4BNP2) - 18H5 (Cat.# 4NT1)
7B5 (Cat. # 4NT1) - 2G9 (Cat. # 4BNP2).

Purification:
Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Other information:
Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.

Related products:
Cat# 4BNP2: BNP, human, antibody 
Cat# 4BFab5: Immune complex 24C5-BNP_proBNP, antibody 
Cat# 8NT1: NT-proBNP recombinant 
Cat# 8NT2: NT-proBNP, recombinant, human  
Cat# 8PRO9: proBNP, recombinant, human 
Cat# 8PRO8: proBNP human recombinant   
Cat# 8GOB2: Human recombinant proBNP glycosylated
Cat# 8BFP: BNP and NT-proBNP Free Plasma
ProBNP, BNP and NT-proBNP Newsletter 

See other Cardiac markers and blood coagulation products

References:
Search NT-proBNP references from PubMed
See other NT-proBNP References