Neuron-specific Enolase (NSE)

DATA SHEET

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Neuron-specific enolase (NSE)
Cat.# 8NS3

Source:
Post mortal human brain.
Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, and syphilis.

Analysis:
Purity > 95 % (SDS-PAGE).

Purification:
Ion-exchange chromatography and gel-filtration.

Activity:
Specific activity is determined for each lot at 25 °C in 45 mM imidazole, pH 6.8, 45 mM KCl, 1.33 mM Mg₂SO₄; 1 mM 2-PG. One unit is defined as the amount of NSE converted 1 mol of 2-phospho-D-glycerate per min at 25 °C (J.Biochem. 87, 1587-1594, 1980). Subunit composition is gamma-gamma.

Immunological activity:
Tested in ELISA and immunoblotting with anti-NSE antibodies.

Presentation:
Solution in 0.1 M Tris-HCl, 5 mM Mg₂SO₄, 0.25 M KCl, pH 5.1 adjusted with sodium phosphate.

Storage:
- 20 °C

Other information:
Avoid repeated freezing and thawing.