DATA SHEET

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PRODUCT NAME: Monoclonal mouse anti-human thyroid stimulating hormone (TSH)
CATALOGUE #: 2TS11

MAbs:
11E4, 7G12, 10C7, 5E8, TSB1, TSB4
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with TSH from human pituitary gland.

Specificity:
MAb 11E4 specifically recognizes β-subunit of human TSH. MAbs 7G12, 10C7 and 5E8 specifically recognize the whole molecule of TSH. MAbs 7G12, 10C7 and 5E8 do not cross-react with human LH, FSH, HCG.
MAbs TSB1 and TSB4 specifically recognize β-subunit of human TSH. MAbs TSB1 and TSB4 do not cross-react with human LH, FSH, HCG.

MAb isotypes:
IgG1 for all MAbs

Applications:
Detection of human TSH in two-site enzyme immunoassay.
Recommended pairs (capture - conjugate): 11E4 - 7G12, 10C7 - 5E8, TSB1 - TSB4 (fab fragments)

Purification:
MAbs 11E4, 7G12, 10C7 and 5E8 by chromatography on protein A Sepharose
MAbs TSB1 and TSB4 by ion exchange chromatography

Presentation:
MAbs 11E4, 7G12, 10C7 and 5E8 in PBS, pH 7.4, 0.1 % sodium azide (NaN3)
MAbs TSB1 and TSB4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.