MasTaq MIX
DATA SHEET
This data sheet is provided for information only.
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MasTaqMIX
Cat.# 7M1
Description:
Ready to use liquid form of premixed ingredients for "hot-start" mode PCR amplification.
Ingredients:
SmarTaq polymerase, each of 4 dNTPs and 10x reaction buffer with MgCl2 and stabilizer.
Final concentrations (when diluted to 50 µl reaction volume): 16.6 mM (NH4)2SO4, 67 mM Tris-HCl (pH 8.8 at 25 °C), 0.01 % Tween 20, 0.2 mM of each dNTP, 2 mM MgCl2, 2.5 U SmarTaq.
Applications:
PCR
Add to 5 µl of MasTaq MIX primers and template and bring the total volume to 50 µl.
Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Storage:
- 20 °C
Mixture is stable even after 150 cycles of freezing and thawing.
Additional product information:
Enzymes and Reagents for molecular biology TechNotes
