DATA SHEET

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PRODUCT NAME: Monoclonal mouse anti-human leptin
CATALOGUE #: 2LE1

MAbs:
3G7, 4F8, 4F12, 5F10, 9C10, 10H1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.

Specificity:
MAbs recognize recombinant human leptin and leptin circulating in human blood.

MAb isotypes:
IgG1 for all MAbs

Applications:
Leptin immunoassay. All MAbs recognize human leptin in Western blotting.
MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity.
Recommended pairs for sandwich immunoassay (capture - conjugate):
                   3G7 - 4F12
                   4F12 - 3G7
                   3G7 - 9C10
                   4F12 - 10H1

Purification:
MAb 4F12: Chromatography on protein A Sepharose and gel filtration,
other MAbs: Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

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References:
Search Leptin references from PubMed
See other Leptin References