Leptin antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human leptin
Cat.# 2LE1
MAbs:
3G7, 4F8, 4F12, 5F10, 9C10, 10H1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.
Specificity:
MAbs recognize recombinant human leptin and leptin circulating in human blood.
MAb isotypes:
IgG1 for all MAbs
Applications:
Leptin immunoassay. All MAbs recognize human leptin in Western blotting.
MAbs were tested in sandwich immunoassay with human blood samples. All recommended pairs recognize antigen in human blood with high sensitivity.
Recommended pairs for sandwich immunoassay (capture - conjugate):
3G7 - 4F12
4F12 - 3G7
3G7 - 9C10
4F12 - 10H1
Purification:
MAb 4F12: Chromatography on protein A Sepharose and gel filtration,
other MAbs: Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:
Adiponection TechNotes
RBP4 TechNotes
References:
Search Leptin references from PubMed
See all Leptin References
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