Lambda free Light Chains antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human free lambda light chains
Cat. # 1L7
MAbs:
3D12
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with mixture of light chains of human immunoglobulin.
Specificity:
Specific for independent constantly expressed epitope of lambda chain.
Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins.
Specific antibody titer is 1/100 000 by indirect ELISA.
MAb isotypes:
IgG2a for MAb 3D12
Applications:
Detection of lambda Bence-Jones proteins in ELISA. MAb 3D12 can be used for coating and as conjugate.
MAb 3D12 can be used for immunoaffinity purification of light chains.
MAb 3D12 works in immunoblotting.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.



