Interferon Alpha, Human, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human interferon alpha
Cat.# 4IF13
MAbs:
C10F5, AE3, AB5
Hybridoma clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human interferon alpha-A.
Specificity:
MAb C10F5 recognizes common epitopes of alpha-A, -F, -D, -I1, -P human interferon subtypes.
MAb C10F5 reacts with different antigenic epitope, than NK-2.YOK MAb (Celltech).
Kd = 2.4 x 10 -10 M.
MAb isotypes:
IgG1 for MAbs C10F5 and AB5
IgG2a for MAbs AE3
Applications:
MAb C10F5 can be used for the detection of recombinant alpha-A, -F, - D, I1, -P interferon subtypes in two-site immunoassay and in affinity purification of recombinant interferons of alpha-A, -F, -D.
MAbs AE3 and AB5 can be used in ELISA and in Western blotting. The recommended pair for sandwich immunoassay (coating - conjugate): AE3 - AB5 (detection limit 5 pg/ml).
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Other information:
Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.
