Insulin, human, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human insulin
Cat.# 2I1
MAbs:
C7C9, D4B8, 7F8, 3A6, 8E2, 7F5
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
Specificity:
Insulin, human
MAb C7C9 reacts with C-terminal pentapeptide of insulin beta-chain. MAbs 7F8, D4B8, 3A6 and 8E2 react with different antigenic determinants.
Kass for MAb 7F8 is 7.5 x 109M-1
Kass for MAb D4B8 is 1.5 x 108M-1
Kass for MAb 3A6 is 8.0 x 108M-1
Kass for MAb 8E2 is 6.0 x 108M-1
Kass for MAb C7C9 is 1.0 x 109M-1
Kass for MAb 7F5 is 1.0 x 109M-1
These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
MAb isotypes:
IgG1, kappa for all MAbs
Applications:
Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (coating - conjugate): 7F8 - D4B8, 3A6 - 8E2.
MAb D4B8 can be used in immunohistochemistry on frozen sections.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:
Rat C-peptide and proinsulin TechNotes
References:
Search Insulin references from PubMed
See all Insulin, Proinsulin, C-peptide References
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Cat# 2IP10: Insulin-Proinsulin, rat-mouse, antibody
Cat# 2P9: Proinsulin, human, antibody
Cat# 2PR8: Rat proinsulin antibody
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