Hepatitis B Virus Surface Antigen, antibody

 

DATA SHEET

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Monoclonal mouse anti-hepatitis B virus
surface antigen (HBsAg)
Cat.# 3HB12

MAbs:
HB5, HB6, HB11, HB12, HB13, Hs33, Hs41

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant HBsAg of ayw subtype.

Specificity:
HBsAg
MAbs are reacting with the following HBsAg subtypes: ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).

MAb isotypes:
IgG1 for MAbs HB11, HB13, HB12, HB6, HB5, Hs41
IgG2a for MAb Hs33

Applications:
Detection of HBsAg in two-site enzyme immunoassay.
Recommended pairs for sandwich immunoassay:
HB12 - HB11, HB5 - HB6, HB13 - HB6, HB12 - HB6, Hs33 - Hs41
Best pair: Hs33 - Hs41, sensitivity 50 pg/ml

Purification:
Chromatography on protein G Sepharose.

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

References:
Search Hepatitis B Virus Surface Antigen references from PubMed 
See all HBsAg References
See references where Monoclonal mouse anti-hepatitis B virus surface antigen (HBsAg) has been used in

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Cat# 8HS7ay: HBsAg, ayw Subtype
Cat# 8HS7-2ad: HBsAg, adw Subtype
Cat# 8HS7-3: HBsAg, adr Subtype 

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