DATA SHEET

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PRODUCT NAME: Monoclonal mouse anti-hepatitis B virus surface antigen (HBsAg)
CATALOGUE #: 3HB12

MAbs:
HB5, HB6, HB11, HB12, HB13, Hs33, Hs41
New MAbs: B5, B1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant HBsAg of ayw subtype (MAbs HB5, HB6, HB11, HB13, HB12, Hs33, Hs41) and with native HBsAg (MAbs B5, B1).

Specificity:
HBsAg
MAbs are reacting with the following HBsAg subtypes: ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).

MAb isotypes:
IgG1 for MAbs HB11, HB13, HB12, HB6, HB5, Hs41 and B1
IgG2a for MAbs Hs33 and B5

Applications:
Detection of HBsAg in two-site enzyme immunoassay.
Recommended pairs for sandwich immunoassay:
HB12 - HB11, HB5 - HB6, HB13 - HB6, HB12 - HB6, Hs33 - Hs41, B1 - B5
Best pair: Hs33 - Hs41, sensitivity 50 pg/ml

Purification:
MAbs B5 and B1 are purified by chromatography on protein A Sepharose. All other MAbs are purified by chromatography on protein G Sepharose.

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Related products:
Cat# 3HA18: Hepatitis A Antigen, antibody  
Cat# 3HB17: Hepatitis B Virus Core Antigen, antibody  
Cat# 3HBe24: Hepatitis B Virus e antigen, antibody   
Cat# 8HS7: HBsAg, ayw Subtype
Cat# 8HS7-2: HBsAg, adw Subtype
Cat# 8HS7-3: HBsAg, adr Subtype 

See other infectious and veterinary reagents  

References:
Search Hepatitis B Virus Surface Antigen references from PubMed 
See other References