Dopamine, antibody

 

DATA SHEET

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Monoclonal mouse anti-dopamine
Cat.# 4D5

MAbs:
2B11
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with dopamine conjugated with bovine serum albumin.

Specificity:
Catecholamine
Affinity of MAb 2B11:
Kd = 10-8 for DA-BSA
Kd = 10-7 for NA-BSA
Kd = 10-4 for unconjugated DA
At 1 µM MAb 2B11 inhibits dopamine binding to D-2 receptor in membrane P-2 fraction from human Corpus striatum.

MAb isotypes:
IgG1 for MAb 2B11

Applications:
Immunocytochemistry detection of catecholamine-secreted cells.

Purification:
Chromatography on protein G Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
 

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