Diphtheria Toxin antibody

DATA SHEET

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Monoclonal mouse anti-Diphtheria toxin
Cat.# 2DT13

MAbs:
1H2, 3B6, DiD1, DiB4, DiH1
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid (MAbs 1H2, 3B6) or supernatant from C. diphtheriae (MAbs DiD1, DiB4, DiH1).

Specificity:
MAbs react with different determinants of Diphtheria toxin and anatoxin. They do not react with free A or B subunits of Diphtheria toxin.

MAb isotypes:
IgG1 for MAbs 1H2, 3B6, DiH1
IgG2b for MAbs DiD1, DiB4

Applications:
Detection of Diphtheria toxin by different immunochemical techniques.
All MAbs are working in EIA. Recommended pairs for sandwich immunoassay (coating – conjugate): 3B6 – 1H2, DiH1 – DiB4, DiH1 – DiD1
MAbs DiD1 and DiB4 are working in Western blotting.

Purification:
MAbs 1H2 and 3B6 by chromatography on protein A Sepharose
MAbs DiD1, DiB4 and DiH1 by chromatography on protein G Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN₃)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.