C-peptide, human, antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-human C-peptide
Cat.# 2I2
MAbs:
1H8, 2B7, 4H8, 5B8, 7E10, 2A11
Hybridoma clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide construction.
Specificity:
No cross-reactivity with human, bovine, porcine and mouse/rat insulin.
Affinity (Kd) > 1 x 108 M for all MAbs.
All MAbs bind with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by MAbs are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.
MAb isotypes:
IgG1, kappa for all MAbs
Applications:
Competitive ELISA or RIA detection of C-peptide in human serum after immunospecific removal of proinsulin (with solid-phased 1D6 or 1D3 MAbs to human proinsulin).
Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum.
Specific immunosorbtional removal of total proinsulin and C-peptide molecules from serum samples (with solid-phase 1H8, 2B7 and 4H8) before the measurement of human insulin.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
