DATA SHEET

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PRODUCT NAME: Monoclonal mouse anti-human brain natriuretic peptide (BNP)
CATALOGUE #: 4BNP2

MAbs:
24C5, 26E2, 2G9, 50E1, 50B7, 57H3 , 43B12
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human synthetic BNP (whole molecule) or synthetic BNP peptide 11FGRKMDRISSSS22 (for MAbs 24C5 and 26E2) conjugated with carrier protein.

Specificity:
Human BNP and proBNP.

MAb isotypes:
IgG1 for MAbs 2G9, 24C5, 50E1, 26E2
IgG2a for MAbs 43B12, 57H3, 50B7

Applications:
BNP and proBNP immunoassay. All MAbs recognize BNP and proBNP in sandwich immunoassay.
MAbs 2G9, 24C5, 26E2, 50E1, 50B7, 57H3 and 43B12 react with proBNP and BNP in Western blotting.
Recommended pairs for BNP sandwich immunoassay (coating - conjugate): 
50E1 - 24C5, 50E1 - 26E2, 24C5 - 50B7, 24C5 - 57H3
Sensitivity of the pair 50E1 - 24C5 is better than 1 pg/ml (synthetic BNP, Bachem). All pairs recognize with high sensitivity the antigen in plasma samples of patients with heart failure.
MAb 50E1 can be used for proBNP detection in pairs with anti-NTproBNP MAbs 16F3 or 18H5 (Cat.# 4NT1).

Purification:
Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Other information:
Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.

Related products:
Cat# 4NT1: NT-proBNP, human, antibody 
Cat# 4BFab5: Immune complex 24C5-BNP_proBNP, antibody 
Cat# 8NT1: NT-proBNP recombinant
Cat# 8NT2: NT-proBNP, recombinant, human  
Cat# 8PRO9: proBNP, recombinant, human   
Cat# 8PRO8: proBNP human recombinant   
Cat# 8GOB2: Human recombinant proBNP glycosylated 
Cat# 8BFP: BNP and NT-proBNP Free Plasma 
ProBNP, BNP and NT-proBNP Newsletter

See other Cardiac markers and blood coagulation products

References:
Search BNP references from PubMed
See other BNP References