Alpha-Fetoprotein (AFP), Human, antibody

 

DATA SHEET

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Monoclonal mouse anti-human alpha-fetoprotein
Cat.# 4F16

New MAb: XA2
5H7, 4A3

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human alpha-fetoprotein.

Specificity:
MAbs react with different determinants of human alpha-fetoprotein.
MAbs do not cross-react with human albumin.

MAb isotypes:
IgG1 for MAb 5H7 and XA2
IgG2a for MAb 4A3

Applications:
We recommend the following pair in sandwich immunoassay (coating - conjugate): 4A3 - 5H7

Purification:
MAbs 5H7 and 4A3 are purified by chromatography on protein A Sepharose
MAb XA2 is purified by ion exchange chromatography

Presentation:
MAbs 5H7 and 4A3 in PBS, pH 7.4, 0.1 % sodium azide (NaN3)
MAb XA2 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 % or 0.05%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
 

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