Troponin T Cardiac antibody
DATA SHEET
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Monoclonal mouse anti-cardiac Troponin T (cTnT)
Cat.# 4T19
MAbs:
9G6, 7F4, 1F2, 7G7, 3D6, 2F3, 1A11, 1C11, 2G3, 1F11, 7A9, 7E7
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT (MAb 2F3, 7G7, 2G3, 1A11, 9G6, 1C11, 1F11, 3D6, 7A9, 7F4), bovine TnT (MAb 1F2) or human Tn complex (MAb 7E7).
Specificity:
MAbs 1F2, 2F3, 7A9, 7F4, 7G7, 2G3, 1A11, 9G6, 1C11, 1F11 and 3D6 have no cross-reaction with skeletal TnT. Cross-reactivity of MAb 7E7 with cardiac TnI is 5 %.
MAb isotypes:
IgG1 for MAbs 1F2, 9G6, 1C11, 3D6, 7A9 and 7G7
IgG2b for MAbs 2F3, 2G3, 1A11, 7F4 and 1F11
IgG for MAb 7E7
Applications:
TnT immunoassay and TnT immunoaffinity purification. It is possible to use following pairs of antibodies for sandwich immunoassay (coating - conjugate): 1C11-9G6, 1C11-7F4, 7G7-9G6, 7G7-1C11, 7G7-2F3, 1C11-7E7
Best capture antibodies for BIAcore are 1C11, 7A9, 9G6, 7G7.
Purification:
Protein A Sepharose chromatography.
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:
Troponin I TechNotes
References:
Search Troponin T references from PubMed.
See all Troponin I References
See references where Monoclonal mouse anti-cardiac Troponin T (cTnT) has been used in
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