Thermus Aquaticus DNA Polymerase, Recombinant
DATA SHEET
This data sheet is provided for information only.
Please contact our Customer Service for further information and pricing.
Thermus aquaticus (Taq) DNA polymerase, recombinant
Cat.# 7T1
Description:
Taq DNA Polymerase (recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus strain YT-1) consists of a single polypeptide chain with a molecular weight 94 kDa. It is a highly processive 5'-3' DNA polymerase lacking 3'-5' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- and exonucleases. This Taq DNA polymerase has a nontemplate-dependent activity, which adds a single deoxyadenosine (A) to the 3' ends of PCR
products (3'-A overhangs). This property allows easily and efficiently to ligate the PCR products in TA cloning vectors. Amplification of target DNA fragments from 100 bp to 4 Kb can be achieved with this enzyme.
Storage and dilution buffer:
20 mM HEPES, pH 7.9, 125 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5 % Tween 20, 50 % glycerol.
Unit definition:
One unit of activity is the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 72 oC.
Reaction buffer 10x (Mg2+ minus):
670 mM Tris-HCl, pH 8.8, 166 mM (NH4)2SO4, 0.1 % Tween 20.
Other common PCR buffers for Taq-DNA polymerases can be used as well.
Optimal concentration of MgCl2 is 1.0-2.5 mM.
Applications:
PCR and DNA labelling.
Quality control:
Activity, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases.
Concentration:
5 units/µl
Storage:
-20 °C
Additional product information:
Enzymes and Reagents for molecular biology TechNotes
References:
See references where Thermus aquaticus (Taq) DNA polymerase, recombinant has been used in
