Recombinant human cardiac troponin T (cTnT)

DATA SHEET

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Recombinant human cardiac troponin T (cTnT)
Cat.# 8RTT5

Synonym names:
Human cardiac troponin T adult isoform, human cardiac troponin T isoform #6 or TnT3

Source: 
Expressed in E. coli

Protein sequence:
Product of gene TNNT2; 288 a.a.r.

Recombinant human cardiac TnT contains additional methionine residue at the N-terminus (in comparison with native protein expressed in cardiomyocytes).

For full sequence, see printable PDF-file.

Application: 
Calibrator or standard in immunoassays, immunogen for antisera production, mass cTnT standard

Analysis: 
Purity > 95 % (SDS-PAGE)
Concentration is determined spectrophotometrically, abs 0.1% (1 g/L) - 0.490 (measured at 280 nm)

Immunological  activity: 
Immunoreactivity is confirmed by reaction with several monoclonal antibodies specific to different epitopes of human cardiac TnT isoform

Purification: 
Immunoaffinity purification followed by ion-exchange chromatography

Presentation: 
Lyophilized from 0.01 M HCl

Reconstitution:
Reconstitute with Tris/urea buffer (20 mM Tris pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol.

Storage: 
Lyophilized -20°C.
Reconstituted -20°C (short term storage) or -70°C (for long term storage).

Other information: 
Avoid repeated freezing and thawing. It is recommended to aliquot the product after reconstitution.

See other Cardiac markers and blood coagulation products