Newcastle disease virus (NDV), antibody

DATA SHEET

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Monoclonal mouse anti-Newcastle disease virus (NDV)
Cat.# 3ND5

MAbs: 9F7, 11F12, 13H3, 9C6, 1C10, 2H4, 8H2
New MAb: 6H12
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Newcastle disease virus (La-sota strain).

Specificity:
MAbs 9F7, 11F12, 13H3, 9C6, 1C10, 2H4 and 8H2 are specific to viral glycoprotein haemagglutinin-neuraminidase.
MAb 6H12 is specific to RNP.

MAb isotypes:
IgG1 for MAb 9F7
IgG2a for MAbs 11F12, 13H3, 9C6, 1C10, 8H2, 6H12
IgM for MAb2H4

Applications:
MAbs 9F7, 11F12, 13H3, 9C6 and 1C10 are working in Western blotting.
MAbs 9F7, 11F12, 13H3, 9C6, 1C10 and 2H4 are working in haemagglutinin inhibition test.
All MAbs are working in ELISA. MAbs 2H4 and 8H2 are recommended as coating antibodies and MAbs 9F7, 11F12 and 1C10 as conjugate antibodies in sandwich immunoassay.
MAb 6H12 is working in Immunofluorescence (IF) and in Peroxidase –Immunohistochemistry staining of infected cells.

Purification:
Chromatography on protein G Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN₃)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.