NT-proBNP recombinant human
DATA SHEET
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Human recombinant NT-proBNP
(amino terminal human brain natriuretic peptide precursor)
Cat.# 8NT2
Source:
Recombinant E.coli
Application:
Calibrator or standard in immunoassays, immunogen for antibody production.
Analysis:
Purity > 95 % (Tricine-SDS-PAGE)
Concentration is determined spectrophotometrically.
Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule.
Predicted molecular mass 8589 Da confirmed by MALDI-MS.
Recombinant human NT-proBNP contains additional methionine residue at the N-terminus (in comparison with native NT-proBNP).
Purification:
Immunoaffinity purification followed by ion-exchange chromatography.
Presentation:
10 mM potassium phosphate, pH 7.4, 150 mM NaCl.
Storage:
- 70 °C
Other information:
Avoid repeated freezing and thawing.
It is recommended to aliquot the product after the first thawing.
To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tris-HCl, pH 7.8, 0.15 M NaCl, 0.01 % Tween 40, 0.5 % bovine serum albumin).
Some applications in which this product may be used are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license, depending on the particular application and country in which the product is used.
Additional product information:
Human ProBNP and proBNP-derived peptides (BNP and NT-proBNP) TechNotes
Antibodies for New Type of BNP immunoassay – “Single Epitope Sandwich” assay TechNotes
References:
Search NT-proBNP recombinant references from PubMed
See all NT-proBNP References
See references where Human recombinant NT-proBNP has been used in
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