MHC Class II (RT-1B) Antigen, antibody

 

DATA SHEET

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Monoclonal mouse anti-rat MHC class II (RT-1B) antigen
Cat.# 5R2

MAbs:
3D6

Hybridoma clone has been derived from hybridization of Ag8.653 myeloma cells with spleen cells of Balb/c mice immunized with purified rat MHC class II proteins.

Specificity:
MHC class II RT-1B antigen
MAb reacts with monomorphic determinant on beta chain of RT-1B molecules. MAb 3D6 does not cross-react with RT-1A,-C,-D rat MHC products.

MAb isotypes:
IgG1, kappa for MAb 3D6

Applications:
MAb 3D6 works in immunofluorescence and flow cytometry, immunoblotting, immunoprecipitation, immunochemistry on frozen sections.
Enumeration of B cells and monocytes.
Localization of B cells/monocytes tissue sections.
Affinity purification of RT-1B antigen of different haplotypes.
Characterization of leukemia and lymphomas.

Purification:
Chromatography on protein A Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
 

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