Legionella Pneumophila LPS, Antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-Legionella pneumophila LPS
Cat.# 3L15
MAbs:
2F10, 5F4
Hybridoma clones have been derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with membrane fraction of Legionella pneumophila, strain Philadelphia 1.
Specificity:
MAbs react with LPS membrane of Legionella pneumophila. MAbs do not cross-react with Legionella pneumophila serogroups 2, 6, 7, 10 and 11, Legionella bozemanii, Legionella lonbeachae, Legionella micdadei, Corynebacterium diphtheria, Pseudomonas fluorescens, Pseudomonas cepacia, Pseudomonas aerugenose, Bordetella pertusis, Leptospira interrogens (Australia, Pomona, Icterogemorrhagia), Toxoplasma gondii, Hemophilus influenza (type B), Hemophilus ducreyi, Brucella abortus, Bacillus anthracis, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium tuberculosis, Neisseria meningitides, Pasteurella multocida, Proteus vulgaris, Shigella sonnei, Staphylocccus faecalis, Streptococcus puogenes, Streptococcus pneumoniae, Yersinia pseudotuberculosis, Yersinia pestis EV76, Salmonella typhi, Escherichia coli K88 and Francisella tularensis 15.
Cross-reaction with Legionella pneumophila serogroups 3 and 4 has not been tested.
MAb isotypes:
IgG3 for MAbs 2F10 and 5F4
Applications:
Detection of Legionella pneumophila in ELISA. Recommended pair for sandwich immunoassay (coating - conjugate): 2F10 - 5F4.
Purification:
Chromatography on protein G Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
References:
See references where Monoclonal mouse anti-Legionella pneumophila LPS has been used in
