Influenza A nucleoprotein, antibody

 

DATA SHEET

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Monoclonal mouse anti-influenza virus type A (nucleoproteid)
Cat.# 3IN5

MAbs: F8
New MAbs: InA108, InA245, InA180, InA224

Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified influenza virus type A strain H1N1.

Specificity:
Influenza virus type A (nucleoprotein).
All MAbs detect NP of Influenza A with high specificity and have no cross reactivity to NP of Influenza B virus.

MAb isotypes:
IgG1 for MAbs InA108 and InA224
IgG2a for MAb F8
IgG2b for MAb InA245
IgG3 for MAb InA180

Applications:
MAb F8 can be used in immunocytochemistry. MAb F8 inhibits viral reproduction after fatty acid acylation.
MAbs InA108 and InA245 detect Influenza A nucleoprotein in Western blotting.
All MAbs are working in ELISA. Recommended pairs for Influenza A nucleoprotein in sandwich ELISA immunoassay (coating – conjugate): InA108 – InA245, InA224 – InA245.
 

Purification:
Chromatography on protein G Sepharose for MAb F8
Chromatography on protein A Sepharose for MAbs InA108, InA245, InA180 and InA224

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Additional product information:
Influenza and other Acute Respiratory Diseases (ARDs) TechNotes

References:
Search Influenza A nucleoprotein references from PubMed
See other Influenza References
See references where Monoclonal mouse anti-influenza virus type A (nucleoproteid) has been used in

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