IgG, antibody

 

DATA SHEET

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Monoclonal mouse anti-human IgG
Cat. # 1G1

New MAb: 5E3
5A9, 3D3, CH1, 2A11
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with IgG from human serum.

Specificity:
Class-specific for human IgG. No allotype restriction. Relatively high affinity.
No cross-reactivity with human IgA, IgM, IgE.
MAbs 5E3, 5A9, 3D3 and CH1 are Fc-region specific.
MAb 2A11 is Fab-region specific

MAb isotypes:
IgG for MAbs 5E3 and 5A9
IgG1 for MAb CH1
IgG2b for MAb 2A11
IgG2a for MAb 3D3

 

Applications:
Detection of specific IgG antibodies and total IgG level.
Recommended pair for sandwich ELISA: 5E3 – 3D3

Purification:
Chromatography on protein G Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. 

References:
See all Immunoglobulins References
See references where Monoclonal mouse anti-human IgG has been usen in

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