Human osteocalcin, antibody

DATA SHEET

This data sheet is provided for information only.
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Monoclonal mouse anti-human osteocalcin
Cat.# 4OC8

MAbs:
3G7, 1C4, 1C7, 3G8, 2H9, 6F9, 8H12
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with bovine osteocalcin with keyhole limpet hemocyanin and recombinant human osteocalcin with glutathione S-transferase.

Specificity:
The ability of the MAbs to recognize full length human osteocalcin, tryptic 1-19, 20-43, synthetic 7-19, 15-31 and bovine osteocalcin was tested with Eu-labelled antigens. See the printable version for further information.

MAb isotypes:
IgG1 for MAbs 1C4, 1C7, 3G8, 6F9, 8H12
IgG2a for MAb 2H9
IgG2b for MAb 3G7

Applications:
MAbs are working in ELISA. Recommended pairs are (coating - conjugate):
3G8 - 2H9 (specific for the intact hOC)
2H9 - 6F9 (detects also the large NH2- terminal fragment)

Purification:
Chromatography on protein A Sepharose

Presentation:
0.9 % NaCl, 0.1 % sodium azide (NaN3)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.