Diphtheria Toxin - A subunit antibody
DATA SHEET
This data sheet is provided for information only.
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Monoclonal mouse anti-Diphtheria toxin, A-subunit
Cat.# 2DT14
MAbs:
8A4, 7F2
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Diphtheria toxoid.
Specificity:
8A4 reacts with free A-subunits of Diphtheria toxin. It does not react with the whole Diphtheria toxin and free B-subunit of Diphtheria toxin.
7F2 reacts with the epitope exposed on free A-subunit and on whole Diphtheria toxin molecule. It does not react with free B-subunit of Diphtheria toxin.
MAb isotypes:
IgG1 for MAb 7F2
IgG2a for MAb 8A4
Applications:
Detection of Diphtheria toxin A-subunit by different immunochemical techniques.
Recommend pair for sandwich immunoassay (coating - conjugate): 8A4 - 7F2.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
