Defensin, Human, antibody

DATA SHEET

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Monoclonal mouse anti-Defensin, Human
Cat.# 4DF8

MAbs:
D1F4, D1C9, D1H5, D2C6, D2B3, D3A6, D3D3, B1F7, B4B6
New MAbs: A6D4, B3C5, B1G8
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic peptide HD1 conjugated with hsp70 (D1F4, D1C9, D1H5), recombinant fuse protein HD2-hsp70 (D2C6, D2B3), recombinant fuse protein HD3-hsp70 (D3A6, D3D3), peptide GRCATRESLSG from human defensin 5 (B1F7), defensin 5 peptide a.a.r. 1-12 (A6D4), defensin 5 peptide a.a.r. 11-21 (B1G8), defensin 5 peptide a.a.r. 16-31 (B3C5) or peptide STRAFTCHCRR from human defensin 6 (B4B6).

Specificity:
MAbs D1F4, D1C9, D1H5, D2C6, D2B3, D3A6, D3D3 all react with cyclic human defensins 1, 2 and 3 (NP1, NP2 and NP3, respectively).
MAbs D1F4, D1C9, D1H5, D2C6, D2B3, D3A6, D3D3 all recognize also linear human defensins 1-3 that are parts of recombinant fuses.
MAbs B1F7, A6D4, B3C5, B1G8 react with defensin 5.
MAb B4B6 reacts with defensin 6.

MAb isotypes:
IgG1 for MAbs D1F4, D1C9, D1H5, D2C6, D2B3, D3A6, D3D3, A6D4
IgG2a for MAb B1G8
IgG2b for MAbs B4B6, B3C5
IgM for MAb B1F7

Applications:
MAbs D1F4, D1C9, D1H5, D2C6, D2B3, D3A6, D3D3 are working in indirect EIA and Western blotting. MAbs D1F4, D1C9 and D1H5 are working also in direct EIA. MAbs B1F7, B4B6, A6D4 and B3C5 are working in EIA and Western blotting. MAb B1G8 is working in EIA.

Purification:
Chromatography on protein G Sepharose

Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN₃)

Storage:
+ 4 °C

Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.