Poster in AACC 2005: NT-proBNP stability & immunoassays using MAbs with different epitope specifity

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A.G. Katrukha¹, A. Tolstaya¹, M. Antopolskii², E. Koshkina³, M. Krasnoselskii³, A. Kharitonov⁴, K. Seferian¹.

NT-proBNP is a useful marker for heart failure, including congestive heart failure. NT-proBNP measurements are becoming routine in clinical practice. But still reports on long term in vitro stability of NT-proBNP molecule are conflicting. Conflicting data could be explained (at least partially) by different epitope specificity of the antibodies utilized in different immunoassays, which are used to assess the stability of the antigen in the sample. The aim of the current study was to reveal the most stable parts of NT-proBNP molecule and epitope specificity of monoclonal antibodies (Mabs). These Mabs could be used for the development of sandwich immunoassay less sensitive to the proteolytic degradation or other modifications of NT-proBNP molecule, which could happen during the long-term storage of blood samples at +6 ºC and at room temperature (+22 ºC).