This data sheet is provided for information only.
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MAbs:
DD1, DD2, DD3, DD4, DD5, DD6, DD22, DD41, DD44, DD46
New MAbs: DD93
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Specificity:
All MAbs recognized D-dimer and high molecular weight fibrin degradation products with different specificities. MAb DD93 recognizing a cross-linked region of D-Dimer. See the printable version for further information.
MAb isotypes:
IgG1 for MAb DD93
IgG2a for MAbs DD1, DD6, DD22, DD41, and DD46
IgG2b for MAbs DD2, DD3, DD4, DD5 and DD44
Applications:
D-dimer and high molecular weight fibrin degradation products immunoassay.
All antibodies recognize D-dimer in ELISA. All MAbs except DD93 recognize D-dimer in Western blotting under non-reducing conditions.
MAbs DD22, DD41, DD44, DD46 interact with ß-chain of D-dimer in Western blotting under reducing conditions.
Recommended pairs to be used in a one-step sandwich immunoassay for D-dimer detection in human plasma (coating - conjugate): DD2 - DD41 and DD2 - DD44.
Recommended pairs to be used in two-step sandwich immunoassay for D-dimer detection in human plasma (coating - detection)*:
DD1 - DD6, DD1 - DD4, DD1 - DD5, DD3 - DD4, DD3 - DD6, DD2 - DD6, DD2 - DD4
and DD2 - DD5
*To be analyzed in a two-step sandwich immunoassay, plasma must be diluted with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage:
+ 4 °C
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.
Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product information:
D-dimer and High Molecular Weight Fibrin Degradation Products TechNotes
References:
Search D-dimer references from PubMed
See all D-dimer References
Related products:
Cat# 8D70; D-dimer
See other Cardiac markers and blood coagulation products
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