Poster in AACC 2010: Sandwich immunoassay prototypes for human LMW adiponectin and for complex of adiponectin with serum albumin
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Sandwich immunoassay prototypes for human LMW adiponectin and for complex of adiponectin with serum albumin
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Alexander E. Kogan 1, Vladimir L. Filatov 1, Ivan A. Katrukha 2, Olga V. Kolosova 3, Oleg A. Nagibin 4, Alexey G. Katrukha 1.
Adiponectin is a protein hormone that belongs to a family of adipokines. Adiponectin is produced by adipocytes and is important regulator of lipid and glucose metabolism. Decreased serum adiponectin might serve as a predictor of future type 2 diabetes mellitus (T2DM) and cardiovascular disorders. Human monomeric adiponectin consists of 244 amino acid residues and has distinct domain structure: it contains both collagen-like and globular C1q-like domains. Native adiponectin exists in the blood stream as a mixture of oligomers: trimers (low-molecular weight form, LMW), hexamers (medium molecular weight form, MMW) and higher order multimers (high molecular weight form, HMW). It is believed that those oligomeric forms exist in the bloodstream as a separate moieties and do not convert into each other. Information about a ratio of oligomeric forms in human blood, about their involvement in Adn hormonal activity and about their possible utilization as markers is contradictive and incomplete. We have developed of a panel of adiponectin-specific monoclonal antibodies (MAbs) and by using such Mabs have studied different Adn forms as well as their possible utilization as diagnostic markers of T2DM.
Conclusions:
1. Panel of monoclonal antibodies specific to different forms of human adiponectin was obtained. Some of these antibodies could be used for the immunodetection of different adiponectin forms in human blood.
2. We’ve demonstrated that LMW Adn-HSA complex exists in human serum.
3. Small-scale clinical studies have shown reduction of LMW Adn and/or LMW Adn-HSA levels in serum of type 2 diabetes patients in comparison with healthy donors.
